Journal: Cancer Genomics & Proteomics

Article Title: Methylation of ESRα Promoters in Benign Breast Tumors Could Be a Signature for Progression to Breast Cancer in African American Women

doi: 10.21873/cgp.20497

Figure Lengend Snippet: Percentage (%) of DNA methylation levels in ESRα P0 and P1 promoters in different types of Caucasian- and African American (AA)-derived human breast cancer (BCa) cell lines and breast tissues.

Article Snippet: Following the manufacturer’s protocol, two hundred-500 ng/µl of the isolated genomic DNA from FPPE tissues was modified using sodium bisulfite treatment EZ Methylation Kit (Zymo Research Inc., Irvine, CA, USA).

Techniques: DNA Methylation Assay

Journal: Cancer Genomics & Proteomics

Article Title: Methylation of ESRα Promoters in Benign Breast Tumors Could Be a Signature for Progression to Breast Cancer in African American Women

doi: 10.21873/cgp.20497

Figure Lengend Snippet: Quantitative DNA methylation of ESRα promoters in different types of breast tissues. Y-axis represents the percentage of cytosine methylation assessed using pyrosequencing; X-axis represents the 5 breast tissue types. Box and whisker plot show the distribution of methylation in the P0 and P1 promoters in the 5 breast tissue types. Boundaries on each box represent values in the 25 th and 75 th percentile. Line within the box marks the median value. Whiskers (error bars) indicate the maximum and minimum value. Among the five breast tissue types, only fibroadenoma showed a significant difference in methylation in the P0 and P1 promoters with the P1 promoters showing high (red arrow) levels of methylation. ER-: ER-negative; ER+: ER-positive.

Article Snippet: Following the manufacturer’s protocol, two hundred-500 ng/µl of the isolated genomic DNA from FPPE tissues was modified using sodium bisulfite treatment EZ Methylation Kit (Zymo Research Inc., Irvine, CA, USA).

Techniques: DNA Methylation Assay, Methylation, Whisker Assay

Journal: Cancer Genomics & Proteomics

Article Title: Methylation of ESRα Promoters in Benign Breast Tumors Could Be a Signature for Progression to Breast Cancer in African American Women

doi: 10.21873/cgp.20497

Figure Lengend Snippet: Quantitation of DNA methylation of ESRα and its expression in BCa cell lines. (A) The bar graph shows the percentage of methylated cytosines in the P0 and P1 promoters of ESRα in BCa cell lines as obtained using pyrosequencing (Y-axis). The X-axis represents the ER-positive (MCF7) and ER-negative (HCC1806, MDA-MB231) BCa cell lines, and the result depicts the mean±standard deviation of two independent experiments. The p-value is indicated for ESRα gene (Mann–Whitney); (B) expression level of ESRα in breast cell lines was assessed using RT- PCR and normalized to MCF7. HCC1806 showed lower expression, while MDA-MB231 showed no expression of ESRα. ER-: ER-negative; ER+: ER-positive.

Article Snippet: Following the manufacturer’s protocol, two hundred-500 ng/µl of the isolated genomic DNA from FPPE tissues was modified using sodium bisulfite treatment EZ Methylation Kit (Zymo Research Inc., Irvine, CA, USA).

Techniques: Quantitation Assay, DNA Methylation Assay, Expressing, Methylation, Standard Deviation, MANN-WHITNEY, Reverse Transcription Polymerase Chain Reaction

Journal: Cancer Genomics & Proteomics

Article Title: Methylation of ESRα Promoters in Benign Breast Tumors Could Be a Signature for Progression to Breast Cancer in African American Women

doi: 10.21873/cgp.20497

Figure Lengend Snippet: Correlation between P0 and P1 ESRα promoter methylation and their expression in human breast tissues. Pearson correlation revealed a negative correlation between methylation level in the P0 promoter and gene expression in mammoplasty and ER-positive tissues.

Article Snippet: Following the manufacturer’s protocol, two hundred-500 ng/µl of the isolated genomic DNA from FPPE tissues was modified using sodium bisulfite treatment EZ Methylation Kit (Zymo Research Inc., Irvine, CA, USA).

Techniques: Methylation, Expressing, Gene Expression

Journal: Cancer Genomics & Proteomics

Article Title: Methylation of ESRα Promoters in Benign Breast Tumors Could Be a Signature for Progression to Breast Cancer in African American Women

doi: 10.21873/cgp.20497

Figure Lengend Snippet: Methylation of ESRα P1 promoter in fibroadenoma, ER-positive and ER-negative BCa tissues. (A) Quantitative DNA methylation analysis in three breast tissue types. Y-axis, percentage of methylated cytosines assessed using pyrosequencing; X-axis, breast tissue types. Box and whisker plot showing the distribution of methylation in the P0 and P1 promoters in the breast tissue types. Boundaries on the box represent values in the 25 th and 75 th percentile. Line within the box marks the median value. Whiskers (error bars) indicates the maximum minimum values; (B) Bar graph comparing the mean methylation levels. ER-: ER-negative; ER+: ER-positive.

Article Snippet: Following the manufacturer’s protocol, two hundred-500 ng/µl of the isolated genomic DNA from FPPE tissues was modified using sodium bisulfite treatment EZ Methylation Kit (Zymo Research Inc., Irvine, CA, USA).

Techniques: Methylation, DNA Methylation Assay, Whisker Assay

Journal: Cancer Genomics & Proteomics

Article Title: Methylation of ESRα Promoters in Benign Breast Tumors Could Be a Signature for Progression to Breast Cancer in African American Women

doi: 10.21873/cgp.20497

Figure Lengend Snippet: Methylation of RASSFIA in fibroadenoma, ER-positive and ER-negative BCa tissues. (A) Quantitative DNA methylation analysis in breast tissues. Y-axis, percentage of methylated cytosines assessed using pyrosequencing; X-axis breast tissue types. Box and whisker plot showing the distribution of RASSF1A methylation in fibroadenoma, ER-positive and ER-negative BCa. Boundaries on the box represent values in the 25 th and 75 th percentile. Line within the box marks the median value. Whiskers (error bars) indicate the maximum and the minimum value; (B) Bar graph comparing the mean methylation levels. ER-: ER-negative; ER+: ER-positive.

Article Snippet: Following the manufacturer’s protocol, two hundred-500 ng/µl of the isolated genomic DNA from FPPE tissues was modified using sodium bisulfite treatment EZ Methylation Kit (Zymo Research Inc., Irvine, CA, USA).

Techniques: Methylation, DNA Methylation Assay, Whisker Assay

Journal: Cancer Genomics & Proteomics

Article Title: Methylation of ESRα Promoters in Benign Breast Tumors Could Be a Signature for Progression to Breast Cancer in African American Women

doi: 10.21873/cgp.20497

Figure Lengend Snippet: Methylation of HIN-1 in fibroadenoma, ER-positive and ER-negative BCa tissues. (A) Quantitative DNA methylation analysis in breast tissues. Y-axis, percentage of methylated cytosines assessed using pyrosequencing; X-axis breast tissue types. Box and whisker plot showing the distribution of HIN1 methylation in fibroadenoma, ER-positive and ER-negative BCa. Boundaries on the box represent values in the 25 th and 75 th percentile. Line within the box marks the median value. Whiskers (error bars) indicate the maximum value and minimum value; (B) Bar graph comparing the mean methylation levels. ER-: ER-negative; ER+: ER-positive.

Article Snippet: Following the manufacturer’s protocol, two hundred-500 ng/µl of the isolated genomic DNA from FPPE tissues was modified using sodium bisulfite treatment EZ Methylation Kit (Zymo Research Inc., Irvine, CA, USA).

Techniques: Methylation, DNA Methylation Assay, Whisker Assay

Journal: Cancer Genomics & Proteomics

Article Title: Methylation of ESRα Promoters in Benign Breast Tumors Could Be a Signature for Progression to Breast Cancer in African American Women

doi: 10.21873/cgp.20497

Figure Lengend Snippet: Interaction between DNA methylation of different genes (ESRα, RASSF1A and HIN-1) within the different types of breast tissues. Bar graph showing mean methylation levels of ESRα, RASSF1A and HIN-1 in fibroadenoma, ER-positive and ER-negative BCa. ANOVA Mixed Model showed a significant interaction between fibroadenoma and ER-positive BCa with increased methylation in the promoter of RASSF1A (>40%) compared to ER-negative BCa ( p 40%). ER-: ER-negative; ER+: ER-positive.

Article Snippet: Following the manufacturer’s protocol, two hundred-500 ng/µl of the isolated genomic DNA from FPPE tissues was modified using sodium bisulfite treatment EZ Methylation Kit (Zymo Research Inc., Irvine, CA, USA).

Techniques: DNA Methylation Assay, Methylation